Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequencing by hybridization by cooperating direct and reverse spectra.

Samuel A Heath1, Franco P Preparata, Joel Young

  • 1Computer Science Department, Brown University, 115 Waterman Street, Providence, RI 02912-1910, USA.

Journal of Computational Biology : a Journal of Computational Molecular Cell Biology
|August 26, 2003
PubMed
Summary

This study introduces tandem spectra for DNA sequencing by hybridization, improving efficiency and nearing theoretical limits. This novel approach offers significant practical advantages for genetic analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Investigation of Unusual <i>N</i>-(Triphenyl-λ<sup>5</sup>-phosphanylidene) Amide Fragmentation Observed upon MS/MS Collision-Induced Dissociation.

Journal of the American Society for Mass Spectrometry·2023
Same author

How is the medical assistance in dying (MAID) process carried out in Nova Scotia, Canada? A qualitative process model flowchart study.

BMJ open·2021
Same author

Efficacy of guanfacine extended release assessed during the morning, afternoon, and evening using a modified Conners' Parent Rating Scale-revised: Short Form.

Journal of child and adolescent psychopharmacology·2014
Same author

On contigs and coverage.

Journal of computational biology : a journal of computational molecular cell biology·2013
Same author

Clinical response and symptomatic remission in short- and long-term trials of lisdexamfetamine dimesylate in adults with attention-deficit/hyperactivity disorder.

BMC psychiatry·2013
Same author

Discontinuity in the transition from pediatric to adult health care for patients with attention-deficit/hyperactivity disorder.

Postgraduate medicine·2012

Area of Science:

  • Genomics
  • Bioinformatics
  • Molecular Biology

Background:

  • DNA sequencing by hybridization (SBH) is a promising alternative to traditional methods.
  • Recent advances have brought SBH performance close to theoretical limits.

Purpose of the Study:

  • To improve the practical performance of SBH algorithms.
  • To evaluate novel probing schemes for enhanced efficiency.

Main Methods:

  • Utilizing a probing scheme based on joint direct and reverse spectra (tandem spectra).
  • Employing a gapped probing pattern for DNA reconstruction.

Main Results:

  • The tandem spectra scheme improves performance per unit microarray area by approximately 5/4.
  • This performance gain appears maximal and not further improvable by adding more spectra.

Related Experiment Videos

Conclusions:

  • Tandem-spectrum reconstruction represents the most effective known technique for sequencing by hybridization.
  • This method offers substantial practical benefits for DNA analysis.