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Inhibiting severe acute respiratory syndrome-associated coronavirus by small interfering RNA
Renli Zhang1, Zhongmin Guo, Jiahai Lu
1Sun Yat-Sen University, Guangzhou 510089, China.
Objective:
To evaluate the effectiveness of small interfering RNA (siRNA) on inhibiting severe acute respiratory syndrome (SARS)-associated coronavirus replication, and to lay bases for the future clinical application of siRNA for the treatment of viral infectious diseases.
Methods:
Vero-E6 cells was transfected with siRNA before SARS virus infection, and the effectiveness of siRNA interference was evaluated by observing the cytopathic effect (CPE) on Vero-E6 cells.
Results:
Five pairs of siRNA showed ability to reduce CPE dose dependently, and two of them had the best effect.
Conclusion:
siRNA may be effective in inhibiting SARS-associated coronavirus replication.