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Gene expression profiles of human bone marrow derived mesenchymal stem cells and tendon cells
Qingliu Hu1, Yingjie Piao, Fei Zou
1Department of Thermal Environmental Medicine, First Military Medical University, Guangzhou 510515, China. huqingliu@163.net
Objective:
To study the gene expression profiles of human bone marrow derived mesenchymal stem cells and tendon cells.
Methods:
Total RNA extracted from human bone marrow derived mesenchymal stem cells and tendon cells underwent reverse transcription, and the products were labeled with alpha-(32)P dCTP. The cDNA probes of total RNA were hybridized to cDNA microarray with 1176 genes, and then the signals were analyzed by Atlas Image analysis software Version 1.01a.
Results:
Fifteen genes associated with cell proliferation and signal transduction were up-regulated, and one gene that takes part in cell-to-cell adhesion was down-regulated in tendon cells.
Conclusion:
The 15 up-regulated and one down-regulated genes may be beneficial to the orientational differentiation of mesenchymal stem cells into tendon cells.