Gene expression profiles of human bone marrow derived mesenchymal stem cells and tendon cells
Related Experiment Video
Updated: Sep 20, 2026
Generation of Mesenchymal Stem Cells from Human Umbilical Cord Tissue and their Differentiation into the Skeletal Muscle Lineage
Published on: August 31, 2022
Qingliu Hu1, Yingjie Piao, Fei Zou
1Department of Thermal Environmental Medicine, First Military Medical University, Guangzhou 510515, China. huqingliu@163.net
Objective:
To study the gene expression profiles of human bone marrow derived mesenchymal stem cells and tendon cells.
Methods:
Total RNA extracted from human bone marrow derived mesenchymal stem cells and tendon cells underwent reverse transcription, and the products were labeled with alpha-(32)P dCTP. The cDNA probes of total RNA were hybridized to cDNA microarray with 1176 genes, and then the signals were analyzed by Atlas Image analysis software Version 1.01a.
Results:
Fifteen genes associated with cell proliferation and signal transduction were up-regulated, and one gene that takes part in cell-to-cell adhesion was down-regulated in tendon cells.
Conclusion:
The 15 up-regulated and one down-regulated genes may be beneficial to the orientational differentiation of mesenchymal stem cells into tendon cells.
