Jove
Visualize
Contact Us

Related Experiment Videos

Age-associated decrease in 8-hydroxy-2'-deoxyguanosine (8-OHdG) immunoreactivity in the autopsied brain.

Makoto Nogami1, Junji Shiga, Noriko Inuzuka

  • 1Department of Legal Medicine, Teikyo University School of Medicine, Tokyo, Japan. mnogami@med.teikyo-u.ac.jp

Legal Medicine (Tokyo, Japan)
|August 26, 2003
PubMed
Summary

8-hydroxy-2'-deoxyguanosine (8-OHdG), a marker of oxidative stress, was studied in human brain autopsies. Findings suggest 8-OHdG levels in glial cells may not reliably indicate infarction injury.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Three cases of drug-related death during treatment for stimulant-induced psychosis.

Legal medicine (Tokyo, Japan)·2026
Same author

Analysis of dextromethorphan concentrations in autopsy samples from two cases of self-poisoning.

Legal medicine (Tokyo, Japan)·2025
Same author

Peripheral alveolar macrophages express more ACE2 immunoreactivity than in hilar area in human autopsied lung.

BMC research notes·2025
Same author

Determination of multiple psychostimulants and antipsychotics in postmortem dried blood spot samples by liquid chromatography-tandem mass spectrometry.

Legal medicine (Tokyo, Japan)·2025
Same author

A case of lacosamide and mirtazapine self-poisoning.

Legal medicine (Tokyo, Japan)·2024
Same author

Simultaneous quantification of 2-aminothiazoline-4-carboxylic acid and 2-aminothiazoline-4-oxoaminoethanoic acid utilizing chemical derivatization followed by liquid chromatography-tandem mass spectrometry.

Journal of pharmaceutical and biomedical analysis·2024
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Neuroscience
  • Biochemistry
  • Pathology

Background:

  • Reactive oxygen species (ROS) modify DNA bases, forming markers like 8-hydroxy-2 -deoxyguanosine (8-OHdG).
  • 8-OHdG generation in the brain is linked to ischemia-reperfusion injury.
  • Investigating 8-OHdG in human brain tissue is crucial for understanding oxidative stress in neurological conditions.

Purpose of the Study:

  • To investigate the presence and distribution of 8-OHdG in human autopsied brain specimens.
  • To determine if 8-OHdG immunoreactivity correlates with infarction and aging in the brain.

Main Methods:

  • Immunohistochemical analysis of 8-OHdG in human brain autopsies.
  • Comparison of 8-OHdG levels in infarcted versus non-ischemic brain regions.
  • Correlation analysis between 8-OHdG immunoreactivity, age, and infarction status.

Related Experiment Videos

Main Results:

  • A significant negative correlation was observed between age and 8-OHdG immunoreactivity in glial cells.
  • 8-OHdG immunoreactivity in glial cells was not elevated in areas surrounding infarction compared to non-ischemic areas.
  • No significant difference in 8-OHdG levels was found in disease-free areas between infarction cases and age-matched controls.

Conclusions:

  • 8-OHdG immunoreactivity in glial cells shows a negative correlation with age in human brains.
  • 8-OHdG may not be a sensitive biomarker for evaluating infarction-induced injury in human autopsied specimens.
  • Further research is needed to clarify the role of 8-OHdG in brain injury and aging.