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DNA sequence analysis of long PCR amplified products at the D1S80 locus
Gotaro Watanabe1, Kenshi Shimizu
1Forensic Science Laboratory, Yamagata Prefectural Police Headquarters, Japan.
Legal Medicine (Tokyo, Japan)
|August 26, 2003
Abstract:
Several PCR amplified products of larger alleles than 41 were observed frequently in D1S80 typing. These long alleles have rarely been analyzed. A total of seven amplified products of larger alleles than 41 at D1S80 were selected for sequence analysis. From the results, all of the samples have a repeat of only 16 base pairs in size, without the first repeat unit. In this study, the largest allele was determined to be the allele 72.