Determination of pancreas injury from bloodstains using human pancreatic elastase III as a marker

A sensitive sandwich enzyme immunoassay for human pancreatic elastase III as a method to identify pancreas injuries from blood or bloodstains was evaluated. The serum levels of human elastase III from healthy adults (n=24) were estimated to be 1.15 +/- 0.6 (+/- SD) ng/ml. The recovery rates of elastase III added to normal human serum were estimated to be 96.3%. Elastase III levels in contents of various digestive organs were assayed and found to be high in the contents of the large intestine. For detection of human elastase III on weapons, samples were prepared from disposable scalpels that had been used to cut skin and skeletal muscle, and then to cut various organs. The mean ratio of elastase III to total protein (ng elastase III/mg protein) on scalpels that had been cut the pancreas (n=11) was 4956+/-3067 (+/- SD), whereas the ratios from other organs were much lower except, in case of several digestive organs. The higher elastase III levels detected in these digestive organs seemed to stem from the amount of intestinal contents adhering to the scalpels. These results suggest that determination of pancreatic elastase III in blood or bloodstains using a sandwich enzyme immunoassay is a very useful and effective tool for identification of pancreas injury in forensic practice.