Intact capillaries sensitive to rate, magnitude, and pattern of shear stress stimuli as assessed by hydraulic conductivity (Lp)

Magnitude of abrupt changes in fluid shear stress (Delta tau) correlates with capillary hydraulic conductivity (Lp). Cultured endothelial cells differentiate between rate of change in and pattern of fluid stimulation; however, neither rate nor pattern sensitivity has been evaluated in individual capillaries. We hypothesized that Lp would be greater following abrupt compared to slow Delta tau and that magnitude of Delta tau would correlate with Lp regardless of rate. Mesenteric venular capillaries (VC, n=41) located in pithed North American leopard frogs (Rana pipiens) were cannulated either above or at in situ pressure to induce abrupt (<0.1 s) or slow (1-5 min) Delta tau. Lp was assessed always at the same pressure (30 cm H2O) using the modified Landis technique. Mean +/- SE Lp was sixfold higher (P=0.005) following abrupt (19.0 +/- 3.9 x 10(-7)) compared to slow (2.7 +/- 0.9 x 10(-7) cm/s/cm H2O) Delta tau after accounting for stimulus pattern variability. Linear relationships between Lp and Delta tau existed for both abrupt [Lp = 0.026(Delta tau)-1.6, R2=0.90, P=0.0001] and slow [Lp = 0.005(Delta tau)-0.3, R2=0.82, P=0.03] stimuli. These results suggest that frog mesenteric VC sense unique characteristics of Delta tau and respond by altering Lp.