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Related Experiment Videos

Disk diffusion-based methods for determining Candida parapsilosis susceptibility to anidulafungin.

Zekaver Odabasi1, Victor Paetznick, Beth P Goldstein

  • 1Division of Infectious Diseases, University of Texas-Houston Medical School, Houston, Texas 77030, USA. zekaver.odabasi@uth.tmc.edu

Antimicrobial Agents and Chemotherapy
|August 26, 2003
PubMed
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Disk diffusion testing for anidulafungin against C. parapsilosis requires specific disk preparation. Optimized methods improve correlation between zone diameters and minimum inhibitory concentrations (MICs).

Area of Science:

  • Mycology
  • Antifungal Susceptibility Testing
  • Clinical Microbiology

Background:

  • Candida parapsilosis is a significant fungal pathogen.
  • Accurate antifungal susceptibility testing is crucial for effective treatment.
  • Disk diffusion is a common method for antimicrobial susceptibility testing.

Purpose of the Study:

  • To evaluate the disk diffusion method for anidulafungin against Candida parapsilosis.
  • To determine optimal conditions for disk diffusion testing of anidulafungin.
  • To compare disk diffusion results with broth microdilution minimum inhibitory concentrations (MICs).

Main Methods:

  • 139 isolates of Candida parapsilosis were tested.
  • Disk diffusion assays were performed using anidulafungin disks.

Related Experiment Videos

  • MICs were determined using NCCLS M27-A2 broth microdilution.
  • Disk preparation involved dissolving anidulafungin in dimethyl sulfoxide and Tween 80.
  • Testing was conducted on Mueller-Hinton agar supplemented with glucose and methylene blue.

    • Main Results:

    • A poor correlation was observed between zone diameters and MICs with standard disk preparation.
    • Optimized disk preparation (1% dimethyl sulfoxide, 0.1% Tween 80) significantly improved the correlation.
    • Testing on supplemented Mueller-Hinton agar enhanced the reliability of the disk diffusion method.

    Conclusions:

    • Disk diffusion can be a reliable method for anidulafungin susceptibility testing of C. parapsilosis with optimized conditions.
    • Specific disk formulation and agar supplementation are critical for accurate results.
    • This optimized method facilitates rapid susceptibility assessment in clinical settings.