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Related Experiment Videos

Coronal microleakage assessed by polymicrobial markers.

Leandro Rodrigues Britto1, Nicholas J Grimaudo, Frank J Vertucci

  • 1Depepartment of Endodontics, University of Florida College of Dentistry, USA. lbritto@dental.ufl.edu

The Journal of Contemporary Dental Practice
|August 26, 2003
PubMed
Summary

Bacterial leakage in root canals is a concern. This study found that while all tested obturation techniques and sealers allowed some bacterial migration, the MicroSeal technique with Ketac-Endo sealer showed the slowest rate.

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Area of Science:

  • Endodontics
  • Microbiology
  • Dental Materials Science

Background:

  • Bacterial leakage in sealed root canals is a significant clinical issue, potentially leading to treatment failure.
  • Previous studies indicate that saliva can facilitate bacterial contamination of endodontically treated teeth.

Purpose of the Study:

  • To evaluate bacterial migration through root canals sealed with different obturation techniques and sealers.
  • To compare the effectiveness of various combinations in preventing microbial contamination.

Main Methods:

  • 144 extracted human mandibular anterior teeth were prepared and divided into two groups (lateral compaction vs. thermomechanical compaction) with five different sealers each.
  • Teeth were inoculated with specific anaerobic bacteria (Peptostreptococcus micros and Prevotella intermedia) over time.

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  • Contamination onset was recorded, and bacterial cultures were used for identification.

    • Main Results:

    • All tested obturation techniques and sealer combinations resulted in bacterial contamination.
    • Statistical analysis (ANOVA) revealed significant differences (p<0.0001) in bacterial migration rates based on the combination of techniques and sealers.
    • The MicroSeal obturation technique combined with Ketac-Endo sealer demonstrated the slowest coronal-apical bacterial migration rate.

    Conclusions:

    • No combination of obturation technique and sealer completely prevented bacterial migration in artificial saliva.
    • The choice of obturation technique and sealer significantly impacts the rate of bacterial leakage.
    • The MicroSeal technique with Ketac-Endo sealer offers a promising approach to reduce bacterial migration in endodontic treatment.