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[Study on the properties of solid substrate room temperature phosphorescence of the antibody labeled with fluorescein
Jia-ming Lui1, Yan Fu, Bing-bin Yu
1Department of Chemistry, Tsinghua University, Beijing 100084, China.
Abstract:
The properties of room temperature phosphorescence (RTP) of the labeled goat anti-human antibody(with fluorescein isothiocynate GAHAb-FITC) and labeled rabit anti-goat antibody(RAGAb-FITC) with FITC in different dilution ratios on many kinds of solid substrates were studied in detail. It is found that on the polyamide membrane, only using Pb(Ac)2 as a heavy atom perturber, both the labeled antibodies and their antigen-antibody conjugated compound with the human immunoglobulin G remained excellent RTP properties of isothiocyanate fluorescein, lambda ex(max)/lambda em(max) = 525/650 nm, the relation of RTP intensity to the concentration was linear in a certain range. The conditions for immunological reaction and RTP emission were optimized. The result means that it is possible to develop a new immunoassay named as solid substrate room temperature phosphorescence immunoassay (SS-RTP-IA), which combines both the specificity of immunological reaction and high sensitivity of luminescence analysis.
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