Related Experiment Video
Updated: Sep 20, 2026

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Published on: March 8, 2020
[Chromatographic behavior of fat-soluble vitamin in reversed phase liquid chromatography with water-isopropanol
Abstract:
The separation of a mixture fat-soluble vitamin A, D, E by reversed-phase HPLC on elution with an isopropanol-water was studied. A procedure was developed for determining vitamin mixtures and was applied to the analysis of 9 multivitamin tablet. The vitamin was fully separated in 15 min using a short C18 column and UV detection at 280 nm. Different solvent were used for sample extraction. The recoveries were measured, and were higher than 95%. Experimental results proved that the three fat-soluble vitamin A, D, E all can be fully eluted by a solution of isopropanol-water in the ratio of 75%:25%.
More Related Videos
11:25Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
Published on: July 11, 2014
05:03Quantitative Analysis of Dietary Vitamin A Metabolites in Murine Ocular and Non-Ocular Tissues Using High-Performance Liquid Chromatography
Published on: December 27, 2024
Related Concept Videos
Chromatography: Introduction
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Elution Process
Thin-Layer Chromatography (TLC): Overview
To begin the analysis, a mixture of compounds is spotted on the starting line on the TLC plate using a thin capillary. The bottom of the...
Gas Chromatography: Types of Columns and Stationary Phases
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
Chromatographic Methods: Terminology