Related Experiment Video
Updated: Sep 20, 2026

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
Published on: December 25, 2016
[Determination of nitrate and nitrite by catalytic spectrophotometry of potassium bromate-methylene blue system]
Ya-li Yuan1, Jian Deng, Jin-sheng Xu
1College of Chemistry and Chemical Engineering, Nanhua University, Hengyang 421001, China.
Abstract:
Nitrate and nitrite is one of important physical and chemical index in the analysis of environmental samples. A new catalytic spectrophotometric method for the determination of nitrate and nitrite has been established based on the catalytic action of nitrite on the oxidative fading reaction of methylene blue with potassium bromate in dilute phosphoric acid medium. The optimum experimental conditions were investigated in detail, including the selection of measuring wave-length and catalytic reactive medium, the dosage of indicator and oxidizer, the influence of temperature and reactive time, and the interference of co-existent ions with the assay. The results showed that, the linear responses of nitrite and nitrate are in the ranges of 0-200 and 0.003-310 micrograms.L-1 respectively. The method offers the advantages of simplicity, rapidity and sensitivity. It has been applied to analyse water samples with satisfactory results.
More Related Videos
Related Concept Videos
Precipitation Titration: Endpoint Detection Methods
In the Volhard method, a standard excess of AgNO3 is first added to the...
Rate-Determining Steps
In a multistep reaction mechanism, one of the elementary steps progresses significantly slower than the others. This slowest step is called the rate-limiting step (or rate-determining step). A reaction cannot proceed faster than its slowest step, and hence, the rate-determining step limits the overall reaction rate.
The concept of rate-determining step can be understood from the analogy of a 4-lane freeway with a short-stretch of traffic-bottleneck caused due to...
Redox Titration: Iodimetry and Iodometry
Precipitation Titration Curve: Analysis
Determining Order of Reaction
Titration of a Weak Acid with a Weak Base
As a result, there is no simple...

