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Published on: February 9, 2012
[Determination of tyrosine based on the fluorescence quenching by molebrum (VI)]
Hai-yan Xiang1, Xiao-ming Chen, Di-wu Zhou
1Printed Circuit Board Factory, Zhuzhou Electric Locomotive Institute, Zhuzhou, 412001 China.
Abstract:
A new selective and sensitive spectrofluorimetric method for the determination of Tyrosine in beer and grape wine has been developed. This method is based on the quenching of fluorescence of Tyrosine by Molybrum(VI). The exitation and emission wavelengths were found to be 278 and 305 nm, respectively. There was a linear relationship in the range of 0.01-14.4 micrograms.mL-1 for Tyrosine. This method is simple and rapid, and has been applied to the determination of Tyrosine in beer and grape wine with satisfactory results.

