[Study on two kinds of antibiotics by the fluorescence thin-layer chromatograph]

Carbostyril medicine is a kind of important chemically synthesized antibiotics, used extensively in the clinic. Ofloxacin (OFXL) and ciprofloxacin (CPLX), being similar in structures, were separated by micellar thin layer chromatography. The 0.0015 mol.L-1 Cetyl trimethyl ammonium bromide (CTAB) and 1% sodium dodecyl sulfate (SDS) (1:1) mixture containing ammonia liquor (1%) and polyamide plates were selected, respectively, as mobile and stationary phases. Various factors affecting the Rf values of drugs on the plates such as the concentration of CTAB, SDS and ammonia liquor, were investigated. Under the appropriate condition, the corresponding Rf is 0.87 and 0.76. The fluorescence determination was carried out at the 280 nm excitation wave-length. Moreover, the stability and the sensibility were measured. The first analytical application of micellar as a mobile phase for the separation of OFLX and CPLX was developed.