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Updated: Aug 16, 2026

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
[Optimization of the solvent system used for thin layer chromatography. VI. Furthering of the uniform design method]
Abstract:
In this paper, we optimized the solvent systems of sulfa drugs and five traditional Chinese medicines for thin layer chromatography by means of uniform design method. The optimal system for the eleven sulfa drugs is benzene-n-butanol-n-hexane (5.71:2.86:1.43). The optimal systems for Mu Xiang and Jiu Li Xiang are all absolute chloroform. The optimal systems for Liang Mian Zhen and San Cha Ku are all acetonitrile-ethyl ether-methyl chloride-cyclohexane (0.05:0.3:0.1:0.55). The optimal system for Jin Yin Hua is ethyl acetate-methyl chloride-formic acid (1:1:1). The method has been born out to be theoretical, simple and practical. In our opinion, it is an effective systematic method.
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