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Related Experiment Videos

Mouse B7-H3 induces antitumor immunity.

X Sun1, M Vale, E Leung

  • 1Department of Molecular Medicine and Pathology, Faculty of Medicine and Health Science, University of Auckland, Auckland, New Zealand.

Gene Therapy
|August 27, 2003
PubMed
Summary

Intratumoral injection of B7-H3 gene therapy eradicated half of EL-4 lymphomas in mice. This treatment generated systemic immunity, mediated by CD8(+) T and NK cells, suggesting B7-H3 as a potential cancer therapy.

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Area of Science:

  • Immunology
  • Cancer Biology
  • Gene Therapy

Background:

  • B7 family proteins regulate immune responses.
  • B7-H3 is expressed on nonlymphoid tissues and may have antitumor properties.
  • B7-H3 can stimulate T-cell responses.

Purpose of the Study:

  • To investigate the potential of B7-H3 as an antitumor agent.
  • To determine if intratumoral B7-H3 gene therapy can eradicate tumors.
  • To identify the immune cells involved in B7-H3-mediated antitumor responses.

Main Methods:

  • Intratumoral injection of a mouse B7-H3 expression plasmid into EL-4 lymphomas.
  • Monitoring tumor growth and regression.
  • Assessing systemic immunity through tumor challenge.

Related Experiment Videos

  • Analyzing the role of CD8(+) T cells, CD4(+) T cells, and NK cells.

    • Main Results:

    • Complete regression of 50% of tumors after B7-H3 plasmid injection.
    • Significant slowing of tumor growth in remaining tumors.
    • Generation of systemic immunity in mice with regressed tumors.
    • B7-H3-mediated immunity involved CD8(+) T cells and NK cells, but not CD4(+) T cells.

    Conclusions:

    • B7-H3 plays a role in cell-mediated antitumor immune responses.
    • B7-H3 gene therapy is a potential therapeutic strategy for cancer.
    • CD8(+) T cells and NK cells are key mediators of B7-H3's antitumor effects.