[Study on the purification of SCAMP and the preparation of its sulfated polysaccharides]

Shark cartilage acid mucopolysaccharide (SCAMP) was extracted from cartilage by the improved method, then we can get SCAMP-F1 and SCAMP-F2 from SCAMP by sepharose-4B chromatography. It was improved that SCAMP-F2 had only one component by sepharose-4B chromatography, PAGE (polyacrylamide gel electrophoresis) and especial centrifuge. At the same time, SCAMP-F2 reacted with sulfated reagents (composed of chlorosulfonic acid and pyridine), and sulfated SCAMP-F2 was obtained. Its sulfur content is 19.6%, higher 6.1% than unsulfated SCAMP-F2, IR and 13C-NMR data showed that additional sulfates were located on the carbon-6.