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Updated: Aug 17, 2026

Application of Stopped-flow Kinetics Methods to Investigate the Mechanism of Action of a DNA Repair Protein
Published on: April 1, 2010
[Studies on the interaction mode between polymyxin B sulfate and DNA with EB as a fluorescence probe]
Chong-qiu Jiang1, Ji-xiang He, Jin-shan Wang
1Shandong Normal University, Jinan 250014, China.
Abstract:
The interaction between polymyxin B sulfate and ct-DNA was studied with ethidium bromide (EB) probe by UV spectroscopy, fluorescence spectroscopy, fluorescent polarization etc. The phenomena of hyperchromism effect and fluorescence quenching proved that polymyxin B sulfate in buffer solution at pH 7.4 can intercalate into the base pairs of ct-DNA. This interaction can cause conformational change of ct-DNA double helix. This interaction can be inhibited in the presence of high concentration of ions. At the same time, PB can combine with the phosphate group of ct-DNA by non-characteristic static force. In this paper, the interaction of PB and ct-DNA was made further discussion.
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