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[Effects of matrine on the relative molecules expression of proliferation and apoptosis in K562 cells]
1Department of Pathology, Fourth Military Medical University, Xi'an 710032, China.
Objective:
To study the effects of matrine on proliferation and apoptosis of K562 cells.
Methods:
Cell culture and cell count, cell cycle phases and number of apoptotic cells were analyzed by flow cytometry. Morphology of apoptotic cells was studied by electron microscopy, BCL-6 and proliferating Cell nuclear antigen (PCNA) were checked by Western blot.
Results:
Matrine could inhibit the proliferation of K562 cells with inhibition rate of 69% after 72 hours matrine induction; number of K562 cells increased significantly in G1 phase, and number of apoptotic cell was 68% at 5th day of matrine treated cells; the typical morphology of apoptotic cells of K562 cells was observed by electron microscopy; BCL-6 was increased and PCNA was decreased in K562 cells induced by matrine.
Conclusion:
Matrine inhibited the proliferation and induced the apoptosis of K562 cells significantly, the mechanism of which might be related to cell cycle arrest and the change of PCNA and BCL-6.
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