Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Gene expression changes after heat shock by cDNA array analysis].

T Y Wang1, N H Wu, Y F Shen

  • 1National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae
|August 28, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Clinical characteristics and prognostic analysis of adult KMT2A-associated acute myeloid leukemia].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2026
Same author

[Clinical efficacy of endoscopic transnasal orbital decompression combined with optic nerve decompression for Graves ophthalmopathy: a retrospective study].

Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery·2026
Same author

[Go ahead bravely and have a brilliant future].

Zhonghua er ke za zhi = Chinese journal of pediatrics·2025
Same author

[Precision strategies for personalized prophylaxis in pediatric hemophilia with coagulation factor and non-factor therapies].

Zhonghua er ke za zhi = Chinese journal of pediatrics·2025
Same author

[The value of coronary angiography-derived fractional flow reserve and coronary angiography-derived index of microcirculatory resistance in coronary artery hemodynamic evaluation].

Zhonghua xin xue guan bing za zhi·2025
Same author

[Efficacy of vacuum sealing drainage in the management of full-thickness incision dehiscence wounds in the perineum after total pelvic exenteration].

Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery·2025

Heat shock alters gene expression in Jurkat cells, increasing heat shock proteins (HSPs), c-Jun, and CLK-1 while decreasing integrin alpha-4 and transforming growth factor beta.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genomics

Context:

  • Heat stress is a critical environmental factor impacting cellular function.
  • Understanding cellular responses to heat shock is vital for various biological and medical fields.

Purpose:

  • To investigate global gene expression changes in Jurkat cells subjected to heat shock.
  • To identify specific genes upregulated or downregulated following thermal stress.

Summary:

  • Total RNA was isolated from Jurkat cells before and after heat shock.
  • cDNA expression arrays were utilized to analyze gene expression profiles.
  • Significant changes were observed, with increased expression of heat shock genes, c-Jun, and CLK-1, and decreased expression of integrin alpha-4 and transforming growth factor beta.

Related Experiment Videos

Impact:

  • Provides insights into the molecular mechanisms of cellular thermotolerance.
  • Identifies key genes involved in the heat shock response pathway.
  • Contributes to the understanding of gene regulation under stress conditions.