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A surface plasmon resonance probe with a novel integrated reference sensor surface.

Takuo Akimoto1, Kazunori Ikebukuro, Isao Karube

  • 1Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1, Komaba, Meguro-ku, Tokyo 153-0031, Japan.

Biosensors & Bioelectronics
|August 28, 2003
PubMed
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This study presents a novel surface plasmon resonance (SPR) sensor with an integrated reference surface. This biosensor enables accurate detection of specific immunoreactions by compensating for nonspecific adsorption, eliminating the need for baseline measurements.

Area of Science:

  • Biomedical Engineering
  • Biosensing Technologies
  • Surface Chemistry

Background:

  • Surface Plasmon Resonance (SPR) is a label-free optical sensing technique widely used for detecting biomolecular interactions.
  • Traditional SPR sensors often require separate reference channels or complex baseline correction procedures to account for environmental fluctuations and nonspecific binding.
  • Developing integrated reference surfaces on SPR probes can significantly simplify experimental protocols and improve detection accuracy.

Purpose of the Study:

  • To develop and characterize a novel SPR sensor probe with an integrated reference surface.
  • To demonstrate the ability of the integrated reference to compensate for nonspecific adsorption and environmental changes.
  • To enable real-time, label-free detection of specific immunoreactions without the need for prior baseline measurements.

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Main Methods:

  • Fabrication of an integrated reference surface on a gold SPR sensor using plasma polymerization of hexamethyldisiloxane to deposit two dielectric layers of different thicknesses.
  • Immobilization of bovine serum albumin (BSA) antigen on a 34 nm dielectric working surface, with an adjacent 1 nm dielectric layer serving as the reference surface.
  • Detection of anti-BSA antibody binding via SPR signal changes and simultaneous monitoring of reference and working surface responses.

Main Results:

  • The SPR sensor successfully detected specific immunoreactions of anti-BSA antibodies.
  • Simultaneous monitoring of reference and working surfaces allowed for real-time determination of the specific binding event.
  • The integrated reference surface effectively compensated for nonspecific adsorption, as confirmed by experiments using anti-human serum albumin antibody.

Conclusions:

  • The developed SPR sensor probe with an integrated reference surface offers a simplified and accurate method for label-free biosensing.
  • This approach enhances the reliability of SPR measurements by mitigating the effects of nonspecific adsorption and environmental variations.
  • The technology holds potential for various diagnostic and research applications requiring sensitive and specific biomolecular detection.