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Published on: April 27, 2011
Identification of white spot syndrome virus latency-related genes in specific-pathogen-free shrimps by use of a
Siti Khadijah1, Soek Ying Neo, M S Hossain
1Animal Health Biotechnology, Temasek Life Sciences Laboratory, 1 Research Link, The National University of Singapore, Singapore 117604.
Abstract:
To investigate whether specific-pathogen-free (SPF) shrimps are asymptomatic carriers of white spot syndrome virus (WSSV), we used a WSSV-specific DNA microarray to measure WSSV gene expression in SPF and WSSV-infected shrimps. Three WSSV genes were found to be relatively highly expressed in SPF shrimps. Reverse transcription-PCR using nested primers as well as real-time detection confirmed that these genes have no detectable counterparts in GenBank; structural analysis of the putative proteins revealed helix-loop-helix and leucine zipper motifs. Viral sequences could be PCR amplified from genomic DNA of SPF shrimp, further supporting the suggestion that these shrimps are asymptomatic carriers.
