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Related Experiment Videos

Expression profiling and gene discovery in the mouse lens.

Michael A Wride1, Fiona C Mansergh, Steffan Adams

  • 1Cardiff University, Cell and Molecular Research Group, School of Optometry and Vision Sciences, Cardiff, Wales, UK. WrideMA@cardiff.ac.uk

Molecular Vision
|August 28, 2003
PubMed
Summary

This study used microarrays to identify 1,668 genes expressed in mouse lenses, revealing novel genes and confirming expression patterns crucial for lens development and function.

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Area of Science:

  • Ophthalmology
  • Genomics
  • Molecular Biology

Background:

  • Cataracts, caused by lens development defects, are surgically treated.
  • The lens serves as a model for studying cellular differentiation and aging.
  • Understanding lens gene expression is key to unraveling its biology and pathology.

Purpose of the Study:

  • To investigate global gene expression patterns in the mouse lens using microarrays.
  • To identify genes preferentially expressed in the lens compared to other tissues.
  • To discover genes differentially regulated in lenses of different ages.

Main Methods:

  • Utilized mouse GeneFilters microarrays (GF400) to analyze lens gene expression.
  • Employed Onto-Express for Gene Ontology analysis of biological processes.

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  • Applied K-means clustering for differential gene expression analysis.
  • Confirmed gene expression using semi-quantitative RT-PCR.

    • Main Results:

    • Identified 1,668 genes expressed in mouse lenses (newborn, 7-day, adult).
    • Characterized gene novelty: 33% known, 7% similar, 24% uncharacterized, 36% novel.
    • Confirmed preferential lens expression and age-dependent differential regulation of specific genes.
    • Detected expression of apoptosis-related genes and hemoglobin isoforms in the lens.

    Conclusions:

    • Provided comprehensive insights into mouse lens gene expression profiles.
    • Identified lens-specific and age-regulated genes essential for future functional studies.
    • Gene expression profiling is a prerequisite for understanding lens development, physiology, and disease.