Related Experiment Videos
Basic fibroblast growth factor high and low affinity binding sites in developing mouse brain, hippocampus and
N A Fayein1, Y Courtois, J C Jeanny
1Unité de Recherches Gérontologiques, INSERM U118, CNRS UA630, Développement et Sénescence Cellulaire, Association Claude Bernard, Paris, France.
Biology of the Cell
|January 1, 1992
Summary
Fibroblast growth factors (FGFs) bind to two receptor types in the developing mouse brain. These binding patterns correlate with neuronal cell development, revealing insights into neurotrophic factor roles.
Area of Science:
- Neuroscience
- Developmental Biology
- Cell Biology
Background:
- Fibroblast growth factors (FGFs) are crucial neurotrophic factors supporting neuron growth and survival.
- Understanding FGF receptor distribution is key to deciphering their role in brain development.
Purpose of the Study:
- To investigate the spatial and temporal distribution of FGF binding sites in the developing mouse brain.
- To identify and characterize different types of FGF receptors and their developmental regulation.
Main Methods:
- Autoradiography was used to map 125I-bFGF binding sites.
- Image analysis quantified binding site distribution across developmental stages.
- Heparitinase sensitivity was employed to differentiate receptor types.
Main Results:
- Two specific bFGF receptor types were identified: heparitinase-sensitive (low affinity, developmentally stable) and heparitinase-resistant (high affinity, developmentally regulated).
- Heparitinase-sensitive receptors localized to basement membranes, potentially serving as a storage form.
- Heparitinase-resistant receptors showed developmental changes, correlating with hippocampal and cerebellar layering in adults and neuroectodermal tissues during embryonic development.
Conclusions:
- FGF receptor distribution patterns in the developing brain are linked to specific cellular processes.
- High-affinity FGF receptors are dynamically regulated during development, coinciding with neuronal proliferation, migration, and differentiation phases.