Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Radiation-induced gene expression in MCF-7 cells.

T Stassen1, M Port, I Nuyken

  • 1Institute of Radiobiology of the German Armed Forces, Neuherbergstr. 11, D-80937 Munich, Germany.

International Journal of Radiation Biology
|August 29, 2003
PubMed
Summary

This study identified six radiation-induced gene targets, including three novel ones, for detecting radiation damage. Gene expression changes over time are crucial for accurate biological dosimetry.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Salivary Gene Expression Changes after Total Body Irradiation in Leukemia Patients: Toward Non-invasive Biodosimetry.

Radiation research·2026
Same author

Using Gene Expression Changes for Monitoring Glioblastoma WHO CNS Grade 4 Recurrence: Validation of NGS Candidate Genes via qRT-PCR.

Radiation research·2026
Same author

Reducing Alcohol Use Among Patients with Hepatitis C.

Substance use & misuse·2026
Same author

Development of a Point-of-Care Microfluidic RNA Extraction Slide for Gene Expression Diagnosis after Irradiation.

Radiation research·2024
Same author

Applicability of Gene Expression in Saliva as an Alternative to Blood for Biodosimetry and Prediction of Radiation-induced Health Effects.

Radiation research·2024
Same author

Validating a Four-gene Set for H-ARS Severity Prediction in Peripheral Blood Samples of Irradiated Rhesus Macaques.

Radiation research·2024

Area of Science:

  • Molecular Biology
  • Radiation Biology
  • Genomics

Background:

  • Biological dosimetry requires sensitive markers for radiation exposure.
  • Early detection of radiation-induced cell damage is critical for medical and safety applications.
  • Gene expression profiling offers a powerful tool to identify such markers.

Purpose of the Study:

  • To detect gene targets for biological dosimetry.
  • To identify early indicators of radiation-induced cell damage.
  • To investigate radiation-induced changes in gene expression patterns.

Main Methods:

  • MCF-7 human mammary carcinoma cells were exposed to X-rays (2 and 6 Gy).
  • Gene expression analysis of 1176 genes was performed using Atlas Human 1.2 Array.

Related Experiment Videos

  • Differential gene expression was validated using quantitative real-time PCR (RTQ-PCR).

    • Main Results:

    • Six dose-dependent radiation-induced genes were identified, with three being novel.
    • Gene expression changes were observed over three days post-irradiation.
    • Analysis revealed activation of cell degradation pathways and inhibition of cell proliferation and repair, suggesting 'active silencing' in radiation-induced cell death.

    Conclusions:

    • Six radiation-induced gene targets, three novel, were identified using gene expression screening.
    • The identified genes show potential for biological dosimetry and early detection of radiation damage.
    • Time-dependent gene expression patterns necessitate consideration for accurate dose assessment, and the 'active silencing' mechanism warrants further investigation.