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Identification of secreted CD155 isoforms.
Béatrice Baury1, Damien Masson, Brian M McDermott
1Institut National de la Santé et de la Recherche Médicale U539, Faculté de Médecine, 1 rue Gaston Veil, 44035 Nantes, France.
Biochemical and Biophysical Research Communications
|August 29, 2003
Summary
Soluble CD155 (sCD155) isoforms exist in biological fluids and reduce poliovirus entry. This suggests a significant role for sCD155 in cellular functions, particularly given its high synthesis levels.
Area of Science:
- Immunology
- Virology
- Molecular Biology
Background:
- CD155 is a member of the immunoglobulin superfamily.
- CD155 plays a role in cell adhesion and immune responses.
- The existence and function of soluble CD155 (sCD155) isoforms are not fully understood.
Purpose of the Study:
- To investigate the presence and characteristics of soluble CD155 (sCD155) isoforms in biological fluids.
- To determine the tissue distribution of CD155 mRNA expression.
- To assess the functional impact of sCD155 on poliovirus entry.
Main Methods:
- Detection and quantification of sCD155 in human serum and cerebrospinal fluid using ELISA.
- Analysis of conditioned media to determine the mechanism of sCD155 release.
- Quantification of CD155 mRNA in human tissues via RT-PCR.
- Assay to measure poliovirus entry mediated by membrane-bound CD155, with and without purified sCD155.
Main Results:
- Soluble CD155 (sCD155) isoforms were detected in conditioned media, human serum, and cerebrospinal fluid.
- sCD155 release does not appear to require protease activity.
- The liver exhibited the highest CD155 mRNA expression, with CD155alpha being the most abundant transcript.
- Purified sCD155 from serum reduced poliovirus entry mediated by membrane-bound CD155.
Conclusions:
- The study confirms the presence of sCD155 in various biological fluids.
- High CD155 synthesis in multiple tissues and the presence of sCD155 suggest a significant physiological role.
- sCD155 may play a role in modulating viral entry, such as poliovirus.
