Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Steroid production in toads.

Luis F Canosa1, Andrea G Pozzi, Cinthia Rosemblit

  • 1PRHOM-CONICET and Laboratorio de Endocrinología Comparada, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II, Ciudad Universitaria, C1428EHA Buenos Aires, Argentina.

The Journal of Steroid Biochemistry and Molecular Biology
|August 29, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Remembering the opponent: neuronal activation associated with social memory in zebrafish.

Frontiers in veterinary science·2026
Same author

Thyroid hormones contribute to JAK/STAT pathway abnormal activation, promoting T-cell lymphoma dissemination.

Blood advances·2025
Same author

Radiosensitization Following Valproic Acid and Gamma Rays in Anaplastic Thyroid Cancer Cells Increases the Expression of hsa-miR-26a-5p.

Archives of medical research·2025
Same author

PKCα Activation via the Thyroid Hormone Membrane Receptor Is Key to Thyroid Cancer Growth.

International journal of molecular sciences·2024
Same author

Editorial: Hormones and receptors in breast cancer.

Frontiers in endocrinology·2023
Same author

Ultrastructural and immunohistochemical characteristics of the olfactory organ cardinal tetra, Paracheirodon axelrodi (Characiformes: Characidae).

Journal of morphology·2022
Same journal

L-Arginine Improves Prenatal and Pre-pubertal Codeine-Induced Steroidogenesis Deregulation by Suppressing Oxidative Stress-Dependent Signaling in Male Wistar Rats.

The Journal of steroid biochemistry and molecular biology·2026
Same journal

Discovery of 17-imidazo[1,2-a]pyridinyl-3α-hydroxy-5α-androstanes (17IPAs) as novel potent positive allosteric modulators of the GABA<sub>A</sub> receptor: Synthesis, in vitro and in silico studies.

The Journal of steroid biochemistry and molecular biology·2026
Same journal

Androgen receptor as therapeutic indicator for LAR-positive triple-negative breast cancer: Preclinical and clinical assessment.

The Journal of steroid biochemistry and molecular biology·2026
Same journal

The 24,25(OH)<sub>2</sub>D<sub>3</sub>-TLCD3B signaling complex and reproductive function in mice and men.

The Journal of steroid biochemistry and molecular biology·2026
Same journal

Testosterone and epitestosterone differentially regulate UDP-glucuronosyltransferases (UGT) activity.

The Journal of steroid biochemistry and molecular biology·2026
Same journal

Interferon-gamma-inducible protein-10 is associated to increased primary bile acids and fibrosis in primary sclerosing cholangitis.

The Journal of steroid biochemistry and molecular biology·2026
See all related articles

In Bufo arenarum, seasonal changes in androgen biosynthesis shift steroidogenesis towards C(21)-steroids, independent of spermiation. This shift is linked to decreased 17-hydroxylase-C(17-20) lyase activity, regulated by FSH and GnRH.

Area of Science:

  • Endocrinology
  • Amphibian reproductive biology
  • Steroidogenesis

Background:

  • Androgen biosynthesis in Bufo arenarum follows a 5-ene pathway, with 5-androstane-3beta,17beta-diol as a testosterone precursor.
  • Steroidogenesis shifts from androgens to C(21)-steroids during the breeding season in B. arenarum.

Purpose of the Study:

  • To investigate the regulation of seasonal changes in testicular steroidogenesis in Bufo arenarum.
  • To determine the role of steroid hormones in hCG-induced spermiation.
  • To elucidate the impact of GnRH and FSH on testicular steroidogenesis.

Main Methods:

  • In vitro analysis of steroidogenesis pathways.
  • Measurement of circulating androgen levels in reproductive animals.
  • Assessment of enzyme activity, including 17-hydroxylase-C(17-20) lyase (CypP450(c17)) and 3beta-hydroxysteroid dehydrogenase (3alphaHSD)/isomerase.

Related Experiment Videos

  • In vitro stimulation with mGnRH and hrFSH.

    • Main Results:

    • Steroid hormones are not involved in hCG-induced spermiation.
    • 17-hydroxylase-C(17-20) lyase (CypP450(c17)) activity decreases during the reproductive season.
    • Circulating androgens are reduced in animals in reproductive condition.
    • Both mGnRH and hrFSH inhibit CypP450(c17) activity in vitro.

    Conclusions:

    • The shift in steroidogenesis during the reproductive period in B. arenarum is not related to spermiation.
    • Decreased CypP450(c17) activity is a key factor in seasonal steroidogenic changes.
    • FSH and GnRH contribute to the regulation of testicular steroidogenesis by decreasing CypP450(c17) activity.