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Related Experiment Videos

Engineering proteins that bind, move, make and break DNA.

Cynthia H Collins1, Yohei Yokobayashi, Daisuke Umeno

  • 1Biochemistry and Molecular Biophysics, California Institute of Technology, mail code 210-41, Pasadena, California 91125, USA.

Current Opinion in Biotechnology
|August 29, 2003
PubMed
Summary
This summary is machine-generated.

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Protein engineering advances enable artificial transcription factors and DNA-modifying enzymes for novel biotechnological applications. Researchers are exploiting DNA polymerase tolerance to mutation for new functionalities.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Protein Engineering

Background:

  • Recent protein engineering has yielded artificial transcription factors and DNA-modifying enzymes with novel specificities.
  • Zinc-finger proteins are key targets for protein design, with significant advances in construction and applications.
  • Innovative strategies like combinatorial and evolutionary approaches are used to engineer enzymes.

Purpose of the Study:

  • To review recent advancements in protein engineering for creating artificial transcription factors and DNA-modifying enzymes.
  • To highlight the development of zinc-finger-based transcription factors and their applications.
  • To discuss the engineering of endonucleases, recombinases, and DNA polymerases for new functions.

Main Methods:

  • Protein engineering techniques, including combinatorial and evolutionary strategies.

Related Experiment Videos

  • Design and construction of zinc-finger-based transcription factors.
  • Modification of DNA polymerases to incorporate non-natural nucleotides or exhibit new activities.

    • Main Results:

    • Successful generation of artificial transcription factors binding new DNA sequences.
    • Creation of engineered endonucleases and recombinases with altered specificities.
    • Exploitation of DNA polymerase mutation tolerance to achieve non-natural activities.

    Conclusions:

    • Protein engineering is rapidly advancing the creation of novel DNA-binding and -modifying proteins.
    • These engineered proteins have broad biotechnological potential, including gene regulation and editing.
    • Further exploration of enzyme active site tolerance may unlock new synthetic biology tools.