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Engineered enzymes for improved organic synthesis.

Karl Hult¹, Per Berglund

¹Department of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Center, SE-106 91 Stockholm, Sweden. per.berglund@biotech.kth.se

Current Opinion in Biotechnology

|August 29, 2003

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Enzyme engineering advances organic synthesis by modifying enzyme mechanisms, substrate specificity, and selectivity. Both rational design and random mutagenesis are effective strategies for applied biocatalysis.

Area of Science:

Biocatalysis
Enzyme Engineering
Organic Synthesis

Background:

Enzymes are increasingly modified for organic synthesis.
Modifications aim to alter reaction mechanisms, substrate specificity, and selectivity.

Purpose of the Study:

To review recent developments in enzyme engineering for applied catalysis.
To highlight strategies for modifying enzyme function, including specificity and enantioselectivity.

Main Methods:

Rational enzyme redesign based on structural knowledge.
Random mutagenesis followed by screening for desired enzyme variants.

Main Results:

Successful modifications include altering catalytic mechanisms and substrate specificity.

Related Experiment Videos

Improved enantioselectivity in kinetic resolutions has been achieved.

Both rational design and random mutagenesis are viable strategies.

Conclusions:

Enzyme engineering significantly enhances the utility of enzymes in applied catalysis.
Recent advancements offer diverse approaches for tailoring enzymes to specific synthetic needs.