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Related Experiment Videos

Recent developments in pyridine nucleotide regeneration.

Wilfred A van der Donk1, Huimin Zhao

  • 1Department of Chemistry, University of Illinois at Urbana-Champaign, 600 S. Mathews Avenue, Urbana, IL 61801, USA. vddonk@uiuc.edu

Current Opinion in Biotechnology
|August 29, 2003
PubMed
Summary
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Cofactor regeneration is essential for using NAD(P)-dependent oxidoreductases in chiral synthesis. Recent advances include solvent-stable enzymes, electrochemical methods, and whole-cell systems, improving preparative applications.

Area of Science:

  • Biocatalysis
  • Organic Synthesis
  • Enzyme Engineering

Background:

  • NAD(P)-dependent oxidoreductases are crucial for synthesizing chiral compounds.
  • High costs of pyridine cofactors necessitate in situ regeneration for preparative applications.

Purpose of the Study:

  • To review and highlight recent advancements in cofactor regeneration methodologies for NAD(P)-dependent oxidoreductases.
  • To discuss novel enzymes and alternative systems for efficient cofactor regeneration.

Main Methods:

  • Review of improved existing regeneration techniques.
  • Exploration of newly discovered solvent-stable dehydrogenases.
  • Investigation of enzymes for regenerating reduced or oxidized cofactor forms.
  • Analysis of electrochemical regeneration methods.

Related Experiment Videos

  • Evaluation of natural and engineered whole-cell systems.

    • Main Results:

    • Development of solvent-stable dehydrogenases for enhanced enzyme activity.
    • Identification of novel enzymes capable of regenerating both reduced and oxidized cofactor forms.
    • Successful application of electrochemical methods for cofactor regeneration.
    • Demonstration of whole-cell systems as viable alternatives to purified enzymes.

    Conclusions:

    • Significant progress has been made in cofactor regeneration, broadening the applicability of NAD(P)-dependent oxidoreductases.
    • New enzymatic and electrochemical approaches offer cost-effective and efficient solutions for chiral synthesis.
    • Whole-cell systems present a promising alternative for large-scale biocatalytic processes.