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Molecular characterization of Brazilian human Giardia duodenalis isolates using isoenzyme and random amplified
Míriam O Rocha1, Maria A Gomes, Adriana O Costa
1School of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. morocha@farmacia.ufmg.br
Abstract:
Isoenzymes and RAPD (random amplified polymorphic DNA) analysis were used to characterize three Brazilian human isolates of Giardia duodenalis and its clones. The Portland-1 strain (ATCC 30888) was included in the study as a reference pattern. Both methods divided the isolates into two main groups, one represented by the Portland-1 strain, the other constituted by the Brazilian isolates, which, in turn, were divided into 2 subgroups. The dendogram constructed with the RAPD data, using seven primers, revealed a great heterogeneity between Brazilian isolates and the Portland-1 strain. There was no relationship to the clinical characteristics of the isolates. Although a lot of similarity has been observed among Brazilian isolates and its clones, individual polymorphism was detected, which could be related to the clonal reproduction of this protozoan.