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Detection of dihydrofolate reductase genes by PCR and RFLP
Margarita M Navia1, Joaquim Ruiz, Javier Sanchez-Cespedes
1Institut Clínic d'Infeccions i Immunología, IDIBAPS. Hospital Clínic, Facultad de Medicina., Barcelona, Spain.
Diagnostic Microbiology and Infectious Disease
|August 29, 2003
Abstract:
The presence of plasmid-encoded trimethoprim resistant dfr genes is the most common mechanism responsible for the acquisition of trimethoprim resistance. The usual method to detect the presence of these genes is hybridization with specific probes. We describe an alternative, faster and easier method, based on PCR amplification and RFLP analysis, to discriminate up to sixteen different dfr genes.

