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Updated: Sep 20, 2026

Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids
Published on: March 17, 2016
Relationship between detection limit and bias of accuracy of quantification of RNA by RT-PCR
Carsten Urban1, Andreas Schweinberger, Michael Kundi
1Baxter Vaccine AG, Biomedical Research Center, Uferstr. 15, 2304, Orth/Donau, Austria.
Abstract:
Evidence is presented demonstrating that the distribution of data obtained applying a given RT-PCR method deviates from a normal distribution depending on the limit of detection. The effect of this is a bias towards higher values and concomitantly a systematic error in respect to the accuracy of the evaluation due to this deviation from normality. In addition, evidence is presented that an evaluation assuming a log-normal distribution is more appropriate.
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