Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Reconstituted fusion pore.

Aleksandar Jeremic1, Marie Kelly, Sang-Joon Cho

  • 1Department of Physiology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA.

Biophysical Journal
|August 29, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Three-dimensional organ-resolved modeling of ultrasound-induced acoustic and thermal effects in the mouse abdomen.

Ultrasonics·2026
Same author

Development and Psychometric Validation of the Career Identity Questionnaire for Vocational School Students.

Behavioral sciences (Basel, Switzerland)·2026
Same author

Impact of Therapeutic Ultrasound on Metabolic Hormone Release from Thyroid Gland.

Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine·2026
Same author

Cartiva or fusion for Hallux Rigidus? A systematic review and meta-analysis.

The Journal of foot and ankle surgery : official publication of the American College of Foot and Ankle Surgeons·2026
Same author

Mechanostimulation of neuroendocrine cells and their clinical relevance.

Molecular and cellular biochemistry·2025
Same author

Ultrasound-induced adiponectin release in subcutaneous adipose tissue: implications for obesity treatment.

Ultrasonics·2025
Same journal

Heterogeneous binding of SARS-CoV2 fusion peptide on complex cellular membranes enhances its fusogenicity.

Biophysical journal·2026
Same journal

Tau protein differentially affects Piezo1 and Kir2.1 channels in brain capillary endothelial cells.

Biophysical journal·2026
Same journal

Emergent Intercellular Junction Stability during Cyclic Tissue Loading.

Biophysical journal·2026
Same journal

Enhanced-Sampling Simulations Reveal Distinct Intermediates in SARS-CoV-2 FSE Pseudoknot Interconversion.

Biophysical journal·2026
Same journal

Structure-based simulations of the full Flock House virus capsid reveal pathways and energetics of an infection-critical peptide externalization event.

Biophysical journal·2026
Same journal

Quantifying the Peripheral Surface Information Entropy from Conformational Ensembles of Globular Protein-Peptide Complexes.

Biophysical journal·2026
See all related articles

This study investigates the structure and function of fusion pores, which are essential for cellular secretion. Researchers successfully isolated and reconstituted these basket-like structures, confirming their role in vesicle fusion and content release.

Area of Science:

  • Cell Biology
  • Membrane Biology
  • Biophysics

Background:

  • Fusion pores (porosomes) are critical structures at the cell plasma membrane facilitating secretory vesicle fusion and content release.
  • Previous studies utilized Atomic Force Microscopy (AFM) and Transmission Electron Microscopy (TEM) to visualize fusion pore morphology and dynamics.
  • t-SNARE proteins were previously localized to the base of fusion pores, suggesting their involvement in the fusion process.

Purpose of the Study:

  • To investigate the detailed morphology, function, and molecular composition of immunoisolated fusion pores.
  • To further elucidate the structural organization and functional capabilities of these secretory machinery components.

Main Methods:

  • Immunoisolation of fusion pores from live secretory cells.

Related Experiment Videos

  • Detailed structural analysis using Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM).
  • Biochemical characterization via immunoblot analysis and functional assays involving reconstituted liposomes.

    • Main Results:

    • TEM provided high-resolution structural details of the fusion pore complex.
    • Immunoblot analysis identified multiple protein isoforms and associated chloride channels within the immunoisolated fusion pore.
    • Reconstituted fusion pores exhibited a characteristic cup-shaped, basket-like morphology and demonstrated functional capacity for vesicle fusion.

    Conclusions:

    • The study provides a comprehensive characterization of the immunoisolated fusion pore, revealing its intricate structure and functional significance.
    • Fusion pores are confirmed to be functional units capable of mediating secretory vesicle fusion.
    • The findings enhance our understanding of the molecular mechanisms underlying cellular secretion.