[Cloning the trpBA-genes from Pseudomonas mendocina and Pseudomonas marginata]

The trpBA genes of Pseudomonas mendocina and P. marginata were cloned in Escherichia coli using pBR322 as a vector. Transcription of the cloned genes took place from their own promoters, in the following order: trpB-->trpA. The latter genes were not linked to the trpF gene and could not be induced by indole glycerol phosphate. Thus, the trpBA cluster of P. mendocina and P. marginata are distinguished from that of P. putida, P. aeruginosa (trpIBA) and P. acidovorans (trpFBA).