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Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
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Published on: December 23, 2020

SARS coronavirus-infected cells in lung detected by new in situ hybridization technique

Noriko Nakajima1, Yasuko Asahi-Ozaki, Noriyo Nagata

  • 1Department of Pathology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.

Japanese Journal of Infectious Diseases
|August 29, 2003
PubMed
Abstract

No abstract available in PubMed .

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In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
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A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
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FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...