Related Experiment Video
Updated: Aug 6, 2026

12:23
Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG
Published on: May 16, 2017
Reconstitution of denatured E. coli alkaline phosphatase with E. coli ribosome
Indian Journal of Biochemistry & Biophysics
|December 1, 1992
Abstract:
E. coli alkaline phosphatase was denatured by physical/chemical means. In vitro reconstitution of this denatured enzyme was assisted by 70S E. coli ribosome, as shown by the recovery of its catalytic competence. Almost total recovery of activity of the totally inactivated enzyme was obtained in presence of equimolar concentration of 70S ribosome at 50 degrees C.

