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Updated: Sep 20, 2026

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
Published on: December 25, 2016
[Spectrophotometric determination of nitrite ion with acridine red based on the nitrosation reaction]
Abstract:
A new spectrophotometric method for the determination of nitrite ion was studied. The method is based on the nitrosation reaction of acridine red with nitrite ion in hydrochloric acid medium. The decolorization of acridine red by the reaction with nitrite was used to spectrophotometric determination at 525 nm. The optimum experimental conditions have been confirmed and 6 mol.L-1 hydrochloric acid is 1.5 mL; 5 x 10(-4) mol.L-1 acridine red is 2.0 mL, the reaction time is 20 min. On the selected conditions, Beer's law is obeyed and the linear range for the determination is 0.025-0.5 microgram.mL-1 NO2-.28 coexistent ions were examined and no serious interference for most of ions was observed. The method has been applied to determine nitrite ion in four water samples with satisfactory results. The relative standard deviation was less than 2.6% for 5 measurements in each water sample. A preliminary relation mechanism is supposed.
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