[Study on unfolding process of neo-trichosanthin in the presence of GdnHCl]

Fluorescence spectroscopy and circular dichroism are effective instruments to determine the chemical unfolding process of protein solution. In this paper, both of them were selected to investigate the unfolding processes of neo-trichosanthin in the presence of GdnHCl. One GdnHCl is added, both spectra of fluorescence and circular dichroism changed remarkedly. The denaturation curve of CD ellipticity meets that of fluorescence intensive excitation at 295 nm very well. The result shows that, in the presence of GdnHCl, the unfolding of neo-trichosantin matches the two-state process well. The model is different from the model of trichosanthin that others reported previously. The mechanism will be further probed later.