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Published on: March 9, 2016
[Activity of Enterococcus faecalis (FK-23) preparation as a biological response modifier]
1Research Institute, Nichinichi Food Co., Mie, Japan.
Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan
|December 1, 1992
Summary
Heat-killed Enterococcus faecalis FK-23 immune preparation activates macrophages and neutrophils in mice. This biological response modifier (BRM) shows potential for immune modulation, similar to OK432.
Area of Science:
- Immunology
- Microbiology
Context:
- Enterococcus faecalis FK-23, a heat-killed bacterial preparation isolated from human feces, was investigated for its biological activities.
- The study utilized a mouse model (C3H/He mice) to assess the in vivo and in vitro effects of FK-23.
Purpose:
- To investigate the biological activity and immune-modulating effects of the Enterococcus faecalis FK-23 preparation.
- To evaluate FK-23's impact on phagocyte activation, specifically neutrophils and macrophages, and cytokine production.
Summary:
- Intraperitoneal injection of FK-23 led to neutrophil and macrophage accumulation in mouse peritoneal cavities.
- FK-23 stimulated macrophages to produce tumor necrosis factor (TNF) in vitro and in vivo, with serum TNF levels reaching 99 U/ml.
- The preparation enhanced neutrophil adherence and active oxygen release but did not induce lymphocyte blastogenesis.
Impact:
- These findings suggest FK-23 acts as a biological response modifier (BRM) with significant phagocyte-activating properties.
- The immune activities observed are comparable to those of the streptococcal antitumor agent OK432, indicating potential therapeutic applications.
