[Determination of ofloxacin in urine by synchronous-derivative fluorimetry]

The internal hormones in human urine emit strong fluorescence and interfere with the determination of trace ofloxacin. However, the experiments indicate that synchronous-first order derivative fluorimetry can be applied to the determination of trace ofloxacin without interference. The linear range is 0.36-3.6 micrograms.mL-1, detection limit is 0.3 microgram.mL-1, RSD is less than 2.5% and the recovery is within the range of 101%-103%.