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Published on: March 7, 2013
[Application of short tandem repeat DNA in model of human-mouse cord blood transplantation]
Xinghui Liu1, Xiaoling Pan, Ning Huang
1Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To evaluate the significance of applying short tandem repeat DNA in the model of human-mouse cord blood transplantation.
Methods:
The techniques of STR-PCR and colony forming cell culture were used to examine the engrafting and influence of hematopoietic growth factor on process in the mice after transplantation of human cord blood (CB).
Results:
1. Human DNA expression was detected in the mice that had received tail-vein injection of human CB (1-5 x 10(7) MNC per mouse) but it was not detected in the mice that have received intraperitoneal injection of human CB. 2. Human CB transplanted in SCID mice could reconstitute the hematopoietic function of the mice; the expression level of human cell was not related with the use of hematopoietic growth factor.
Conclusion:
STR-PCR analysis provide a simple and accurate indication of engraftment in the model of human-mouse cord blood transplantation.
