Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sample filtration, concentration, and separation integrated on microfluidic devices.

B Scott Broyles1, Stephen C Jacobson, J Michael Ramsey

  • 1Oak Ridge National Laboratory, P.O. Box 2008, Oak Ridge, Tennessee 37831-6142, USA.

Analytical Chemistry
|September 2, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Efficient septum formation is essential for chromosome segregation in <i>Bacillus subtilis</i> when SMC function is impaired.

bioRxiv : the preprint server for biology·2026
Same author

Constitutive, endogenous, fluorescent membrane reporters for dynamic cell cycle analysis in <i>Bacillus subtilis</i>.

bioRxiv : the preprint server for biology·2026
Same author

Polyanionic Sugars Drastically Affect Assembly of Human Papilloma Virus Virus-Like Particles.

ACS infectious diseases·2025
Same author

Effective Diameters of Virus-Like Particles Measured by Resistive-Pulse Sensing on In-Plane Nanopore Devices.

Analytical chemistry·2025
Same author

Speed Matters: Directed Assembly of Icosahedral HPV Virus-Like Particles.

Journal of the American Chemical Society·2025
Same author

Modular Anti-Counterfeit Tags Formed by Template-Assisted Self-Assembly of Plasmonic Nanocrystals and Authenticated by Machine Learning.

Advanced functional materials·2025
Same journal

The ACS at 150: The History of Analytical Chemistry Publications and a Century of Progress.

Analytical chemistry·2026
Same journal

Machine Learning-Enabled Image Analysis of Complex Chemical Mixtures: Synthetic Urine Droplets as a Test System.

Analytical chemistry·2026
Same journal

H<sub>2</sub>O<sub>2</sub>/Viscosity Tandem-Locked Fluorescent Probes Based on an In Situ Fluorophore Synthesis Strategy for Colitis Imaging and Diagnosis.

Analytical chemistry·2026
Same journal

TopoStitcher: A Geometric-Topological Structure-Guided Stitching Framework for Single-Molecule Localization Microscopy.

Analytical chemistry·2026
Same journal

Noninvasive SERS Immunosensing of Tyrosinase for Melanoma Monitoring via Microneedle Sampling Integrated with Satellite-Structured Bifunctional Nanozymes.

Analytical chemistry·2026
Same journal

Label-Free Electrochemical CRISPR Platform Gated by Allosteric Transcription Factors for Ultrasensitive Small-Molecule Detection.

Analytical chemistry·2026
See all related articles

This study demonstrates microfabricated devices for integrated sample filtration, solid-phase extraction, and chromatographic separation. These advanced chips enable rapid analysis of polycyclic aromatic compounds with enhanced sensitivity.

Area of Science:

  • Analytical Chemistry
  • Microfluidics
  • Separation Science

Background:

  • Traditional analytical methods often involve multiple complex steps.
  • Microfluidic devices offer miniaturization and integration of analytical processes.
  • Need for efficient sample preparation and separation in complex matrices.

Purpose of the Study:

  • To demonstrate a microfabricated device integrating filtration, solid-phase extraction (SPE), and chromatography.
  • To evaluate the performance of the integrated system for analyzing polycyclic aromatic compounds (PACs).
  • To assess the efficiency of sample concentration and chromatographic separation.

Main Methods:

  • Fabrication of microfluidic chips with integrated sample inlet filtration.
  • Coating of separation channels with a C18 phase for solid-phase extraction and chromatography.

Related Experiment Videos

  • Utilized isocratic, step, and linear gradient elution for solvent programming.
  • Employed open channel electrochromatography for separation of PACs.

    • Main Results:

    • Demonstrated effective sample filtration using an array of microchannels.
    • Achieved significant signal enhancement (400x) for PACs via solid-phase extraction.
    • Resolved four PACs within 50 seconds using open channel electrochromatography.
    • Chip performance remained stable despite the presence of silica particles.

    Conclusions:

    • Microfabricated devices can successfully integrate multiple analytical functions.
    • The developed system provides efficient sample concentration and rapid chromatographic separation.
    • This technology shows promise for sensitive and fast analysis of PACs.