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Mouse blastocyst vitrification compared with the conventional slow-freezing method.

W Tharnprisarn1, S Suwajanakorn, W Sereepapong

  • 1Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.

Journal of the Medical Association of Thailand = Chotmaihet Thangphaet
|September 2, 2003
PubMed
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Vitrification and slow-freezing showed similar mouse blastocyst survival and hatching rates after cryopreservation. Vitrification may be a cost-effective method for preserving excess blastocysts for transfer.

Area of Science:

  • Reproductive Biology
  • Cryobiology
  • Embryology

Background:

  • Cryopreservation is essential for preserving embryos in assisted reproductive technologies.
  • Vitrification and slow-freezing are two primary methods for cryopreserving embryos.
  • Evaluating the efficacy of these methods for blastocyst-stage embryos is crucial.

Purpose of the Study:

  • To compare the survival and hatching rates of mouse blastocysts cryopreserved using vitrification versus slow-freezing.
  • To assess the suitability of vitrification as an economic method for blastocyst cryopreservation.

Main Methods:

  • One hundred and forty-eight in vitro mouse blastocysts were randomly assigned to either vitrification or slow-freezing.
  • Cryopreserved blastocysts were thawed and assessed for survival rates.

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  • Hatching rates of the surviving blastocysts were also evaluated.

    • Main Results:

    • No significant difference was observed in blastocyst survival rates between vitrification (68.33%) and slow-freezing (65.52%) (p = 0.89).
    • Hatching rates were also comparable, with vitrification yielding 51.22% and slow-freezing yielding 44.74% (p = 0.64).
    • These findings indicate similar efficacy for both cryopreservation methods at the blastocyst stage.

    Conclusions:

    • Vitrification is a viable and potentially economic alternative to slow-freezing for cryopreserving mouse blastocysts.
    • This method could be beneficial for centers routinely transferring blastocysts.
    • Further research may explore long-term viability and developmental potential.