Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Antimicrobial peptides containing arginine.

M G Ryadnov1, O V Degtyareva, I A Kashparov

  • 1Institute of Protein Research, Russian Academy of Sciences, Pushchino 142290, Moscow Region, Russia.

Biochemistry. Biokhimiia
|September 2, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A microfluidic platform for the characterisation of membrane active antimicrobials.

Lab on a chip·2019
Same author

Dataset concerning GroEL chaperonin interaction with proteins.

Data in brief·2016
Same author

Affinity chromatography of chaperones based on denatured proteins: Analysis of cell lysates of different origin.

Protein expression and purification·2015
Same author

[Photo-induced processes and reaction dynamics in bacteriorhodopsin].

Biofizika·2015
Same author

Modeling and integral X-ray, optical, and MRI visualization of multiorgan metastases of orthotopic 4T1 breast carcinoma in BALB/c mice.

Bulletin of experimental biology and medicine·2015
Same author

Stable isotope imaging of biological samples with high resolution secondary ion mass spectrometry and complementary techniques.

Methods (San Diego, Calif.)·2014

Synthesized tetradecapeptides exhibit potent antibacterial activity against Escherichia coli, comparable to natural antimicrobial peptides. These novel peptides demonstrate selectivity, showing no harm to human cells or rat thymocytes.

Area of Science:

  • Biochemistry
  • Peptide Synthesis
  • Antimicrobial Research

Background:

  • Antimicrobial peptides (AMPs) are crucial in innate immunity.
  • Developing synthetic AMPs with enhanced efficacy and selectivity is a key research area.
  • Understanding structure-activity relationships of AMPs can guide novel drug design.

Purpose of the Study:

  • To synthesize novel tetradecapeptides with potential antimicrobial properties.
  • To evaluate the antibacterial activity of these synthetic peptides against Escherichia coli.
  • To assess the selectivity of the synthesized peptides towards human and rat cells.

Main Methods:

  • Solid-phase peptide synthesis using Fmoc-amino acids.
  • Determination of minimum inhibitory concentration (MIC) against Escherichia coli.

Related Experiment Videos

  • Cytotoxicity assays using human erythrocytes and rat thymocytes.
  • Circular dichroism spectroscopy to analyze peptide secondary structure in solution.

    • Main Results:

    • Four tetradecapeptides, including (RLARLAR)2, D-(RLARLAR)2, (RLARLAA)2, and (RLGRLGR)2, were successfully synthesized.
    • MIC values ranged from 1 to 12 micro M against Escherichia coli, comparable to natural AMPs.
    • No hemolytic activity against human erythrocytes or toxicity towards rat thymocytes was observed.
    • Peptides adopted alpha-helical structures in 30% trifluoroethanol and 2.5 mM SDS, indicating amphipathic properties.

    Conclusions:

    • The synthesized tetradecapeptides possess significant antibacterial activity against Escherichia coli.
    • These peptides demonstrate a favorable safety profile, lacking toxicity to host cells.
    • The amphipathic alpha-helical structure is likely crucial for their antimicrobial function.