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Coincident pre- and postsynaptic activity modifies GABAergic synapses by postsynaptic changes in Cl- transporter

Melanie A Woodin1, Karunesh Ganguly, Mu-ming Poo

  • 1Division of Neurobiology, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.

Neuron
|September 2, 2003
PubMed
Summary

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GABAergic synapses can be modified by coincident neural activity, altering synaptic strength. This process involves calcium influx and reduces inhibitory signaling, adapting inhibition based on neural firing patterns.

Area of Science:

  • Neuroscience
  • Synaptic Plasticity
  • Neurophysiology

Background:

  • Long-term synaptic modifications are typically associated with glutamatergic synapses.
  • The plasticity of GABAergic synapses and their response to coincident activity remain less understood.

Purpose of the Study:

  • To investigate if GABAergic synapses exhibit plasticity in response to coincident pre- and postsynaptic activation.
  • To elucidate the molecular mechanisms underlying this observed synaptic modification.

Main Methods:

  • Experiments were conducted using hippocampal cultures and acute hippocampal slices.
  • Repetitive postsynaptic spiking was paired with GABAergic synapse activation.
  • Calcium influx and K+-Cl- cotransporter activity were measured.

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Main Results:

  • Repetitive postsynaptic spiking before or after GABAergic synapse activation induced a persistent change in synaptic strength.
  • This modification was dependent on calcium (Ca2+) influx via postsynaptic L-type Ca2+ channels.
  • A decrease in K+-Cl- cotransport activity was identified as the mechanism, reducing inhibition.

Conclusions:

  • GABAergic synapses demonstrate plasticity, responding to coincident pre- and postsynaptic spiking.
  • This plasticity modulates the strength of inhibition based on the temporal patterns of neuronal activity.
  • The findings reveal a novel mechanism for regulating inhibition in the hippocampus.