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Related Experiment Videos

High throughput protein production for functional proteomics.

Pascal Braun1, Josh LaBaer

  • 1Harvard University, Department for Chemistry and Chemical Biology, 12 Oxford Street, Cambridge, MA 02138, USA. pbraun@hms.harvard.edu

Trends in Biotechnology
|September 2, 2003
PubMed
Summary
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Genome projects enhance human health by improving protein function understanding. This necessitates developing high-throughput (HT) protein expression systems for large-scale proteomic research and therapeutic applications.

Area of Science:

  • Genomics and Proteomics
  • Molecular Biology
  • Biotechnology

Background:

  • Genome projects provide vast protein-coding sequences, driving the need for functional studies.
  • Proteins are crucial biological entities and targets for nearly all pharmaceuticals.
  • The post-genomic era demands high-throughput (HT) methods for protein investigation.

Purpose of the Study:

  • To address the need for scalable protein isolation methods.
  • To explore approaches for developing proteome-scale protein expression systems.
  • To facilitate systematic and large-scale investigation of protein function.

Main Methods:

  • Developing high-throughput (HT) protein expression systems.
  • Implementing strategies for large-scale protein isolation.

Related Experiment Videos

  • Utilizing novel protein-coding sequences and cDNA collections.

    • Main Results:

    • Identification of challenges in developing proteome-scale protein expression.
    • Description of approaches to overcome these challenges.
    • Laying the groundwork for systematic proteomic research.

    Conclusions:

    • Effective proteome-scale protein expression is essential for advancing human health.
    • HT protein isolation methods are critical for post-genomic research.
    • Continued development of protein expression systems will unlock new therapeutic avenues.