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Updated: Sep 20, 2026

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion
Published on: February 10, 2023
Streptomyces lividans contains a minimal functional signal recognition particle that is involved in protein secretion
Arantxa Palacín1, Ricardo de la Fuente1, Inmaculada Valle1
1Centro Nacional de Biotecnología, Campus de la Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain.
Abstract:
The bacterial version of the mammalian signal recognition particle (SRP) is well conserved and essential to all known bacteria. The genes for the Streptomyces lividans SRP components have been cloned and characterized. FtsY resembles the mammalian SRP receptor and the S. lividans SRP consists of Ffh, a homologue of the mammalian SRP54 protein, and scRNA, which is a small size RNA of 82 nt in length. Co-immunoprecipitation studies confirmed that Ffh and scRNA are probably the only components of the S. lividans SRP and that pre-agarase can co-immunoprecipitate with Ffh, suggesting that the SRP is involved in targeting secretory proteins.
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