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Related Experiment Videos

Flow rate calibration II: a clinical evaluation study using PanLeucoGating as a single-platform protocol.

Ian Storie1, Alex Sawle, Liam Whitby

  • 1UK NEQAS for Leucocyte Immunophenotyping, Royal Hallamshire Hospital, Sheffield, United Kingdom.

Cytometry. Part B, Clinical Cytometry
|September 2, 2003
PubMed
Summary

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A new flow-rate based method makes PanLeucoGating a cost-effective single-platform approach for accurate CD4(+) T-lymphocyte counts in HIV monitoring. This method maintains precision and affordability for clinical use.

Area of Science:

  • Immunology
  • Virology
  • Biotechnology

Background:

  • CD4(+) T-lymphocyte enumeration is crucial for monitoring human immunodeficiency virus (HIV) progression.
  • Current methods require cost-effective, accurate, and precise protocols.
  • PanLeucoGating offers potential but needs accurate total white cell count for single-platform use.

Purpose of the Study:

  • To evaluate a novel flow-rate based calibration method for the PanLeucoGating protocol.
  • To establish PanLeucoGating as a single-platform (SP) approach for CD4(+) T-lymphocyte enumeration.
  • To assess the cost-effectiveness, accuracy, and precision of this new SP method.

Main Methods:

  • Analyzed 113 HIV samples using three protocols: predicate SP bead-based method, dual-platform PanLeucoGating, and the new flow-rate based SP PanLeucoGating.

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  • Demonstrated ease of flow rate calibration.
  • Compared the new SP approach against the state-of-the-art SP method.

    • Main Results:

    • High correlation (r(2) = 0.9928) observed between predicate SP and SP PanLeucoGating for CD4 counts.
    • Minimal bias (8 cells/microl overall, 0 cells/microl in the 0-200 range).
    • Reduced cost to approximately US $1 per CD4(+) T-lymphocyte count for batched samples.

    Conclusions:

    • The SP PanLeucoGating, utilizing flow-rate calibration, is a viable and cost-effective method for CD4(+) T-lymphocyte enumeration.
    • This approach maintains the accuracy and precision required for HIV monitoring.
    • The method presents a significant advancement in affordable HIV diagnostics.