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Related Experiment Videos

Coordinated and conserved expression of alphoid repeat and alphoid repeat-tagged coding sequences.

Yin-Xiong Li1, Margaret L Kirby

  • 1Department of Pediatrics, Duke University Medical Center, Durham, North Carolina 27710, USA.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|September 2, 2003
PubMed
Summary

Researchers discovered an alpha-like DNA repeat sequence with differential expression during early embryogenesis in chick and zebrafish. This repeat may function as a gene regulatory control element in vertebrate development.

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Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • Simple sequence DNA repeats are common in genomes.
  • Their role in embryonic gene regulation is not fully understood.

Purpose of the Study:

  • To investigate the expression patterns and potential function of an alpha-like simple sequence DNA repeat during early vertebrate embryogenesis.
  • To identify specific transcripts associated with this repeat sequence.

Main Methods:

  • Differential expression analysis of alphoid repeat transcripts in chick and zebrafish embryos.
  • Identification of alphoid repeat-tagged mRNA (ESalphaT) using expressed sequence tag databases, RACE, and cDNA library screening.
  • Localization of the alphoid element within ESalphaT sequences.

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Main Results:

  • Alphoid repeat transcripts were ubiquitously expressed early in embryogenesis, then restricted to cardiac neural crest, head, and heart tissues.
  • Two types of transcripts, alphoid repeat RNA and ESalphaT, were identified and coordinately expressed.
  • ESalphaTs encoded various proteins and were expressed in tissues positive for alphoid repeat RNA.

Conclusions:

  • The conserved, differential expression of alphoid-like repeat transcripts suggests a role as a gene regulatory control element.
  • This repeat sequence may be involved in the gene regulation network of vertebrate embryonic development.
  • The findings provide insights into the function of simple sequence repeats in developmental processes.